Research #2

DAYA HAMBAT EKSTRAK KASAR SENYAWA DAUN KUMIS KUCING (Orthosiphon aristatus) TERHADAP PERTUMBUHAN Pseudomonas aeruginosa

Profita Oktavina Maharani, Biology Faculty, Jenderal Soudirman University

ABSTRACT

Pseudomonas aeruginosa is a kind of negative Gram bacteria with characteristic of opportunistic that could cause infection. Infection type that occurred among other things urine-duct infection, respiratory infection, dermatitis, smooth tissue infection, bone and joint infection, digestive infection, and vary of systemic duct infection. Orthosiphon aristatus is the one of plantation that contain chemical with characteristic of antibacterial to bacteria which could cause infection. It has been conducted an experiment to find out the class of active compound that contain in crude extract of O. aristatus leaf which able to inhibit P. aeruginosa and the Minimum Inhibition Concentration (MIC). This research was carried out at Microbiology Laboratory Biology Faculty of Jenderal Sudirman University on October 2008 – January 2009. The research used completely randomized design with 6 treatments and thrice replications. Each treatment used concentration of O. aristatus extract 0% (K₀), 2.5% (K₁), 5% (K₂), 7.5% (K₃), 10% (K₄) and 12.5% (K₅), respectively examined to P. aeruginosa. Examination was carried out by gel dilution method and calculation of total bacteria colony used Total Plate Count (TPC) method. The substance of antibacterial is alkaloid and flavonoid. MIC crude extract of O. aristatus to P. aeruginosa was equal to 2.5%.

Key words: O. aristatus, P. aeruginosa, infection, antibacterial, MIC